The Collaborative Sequencing Center

About Us

Founded in 2010 at the Translational Genomics Research Institute (TGen) in Phoenix, Arizona, the Collaborative Sequencing Center (CSC) provides advanced short read and long read sequencing services for projects of any scale. We offer a wide range of applications, including whole genome sequencing, telomere to telomere assemblies, exome sequencing, RNA sequencing, and full-length transcript sequencing. Our team has extensive expertise in nucleic acid extraction and library preparation for a variety of workflows.

Our facility features state-of-the-art platforms including the Illumina NovaSeq X Plus and PacBio Onso for short read sequencing, and the Oxford Nanopore PromethION and PacBio Revio for long read sequencing. Every project is supported by our internally developed laboratory information management system, which ensures seamless tracking from sample receipt through quality control, sequencing, and comprehensive post-run data review.

Sequencing Capabilities

Next Generation Sequencing Platforms

Illumina NovaSeqX Plus

      • 10B Lane: ~1.25B read clusters (~2.5B PE read clusters)
      • 25B Lane: ~3.125B read clusters (~6.25B PE read clusters)

PacBio Onso

Long-Read Third Generation Sequencing Platforms

Oxford Nanopore PromethION

      • Includes up to three loadings over 72 hour run

PacBio Revio

High-Throughput Instrumentation

Nucleic Acid Isolation

Thermo Scientific KingFisher Apex System

Automated Library Preparation Platforms

Agilent Bravo NGS Workstation

Hamilton NGS Star MOA

Quality Control

Agilent Tapestation 4200

Agilent FemtoPulse

Thermo Scientific Varioskan LUX Microplate Reader

Service Overview

Nucleic Acid Isolation

 

High-Throughput DNA Isolations using the Thermo Scientific KingFisher Apex System

      • Blood/Cells/Buffy Coat
      • Fresh/Frozen Tissues

High-Throughput RNA Isolations using the Thermo Scientific KingFisher Apex System

      • PAXgene Blood Tubes
      • Fresh/Frozen Tissues
      • Cells

High-Throughput Library Preparations

 

Whole Genome Sequencing

      • PCR-Free
      • Amplified

Targeted Sequencing

Transcriptome Sequencing

      • mRNA
      • Full Length RNA via PacBio Kinnex
      • Ribosomal/Globin Depleted RNA

 

Location & Hours of Operation

Location Hours
445 N. 5th Street
Phoenix, AZ 85004
Monday-Friday
9am-4:30pm

For any inquiries about requests for estimates, services, equipment, or training, please email thecsc@tgen.org.

 

Leadership

Jonathan Keats, Ph.D
Assistant Professor, Cancer Genomics and Therapeutics Division
Director of Bioinformatics and Collaborative Sequencing Center
Scientific Director, Judy and Bernard Briskin Center for Multiple Myeloma Research
 
Shari Kyman
Lab Manager, Collaborative Sequencing Center
 
Daniel Quezada Enriquez
Bioinformatician II, Collaborative Sequencing Center
 

Links & Resources

Initiating a Service Request

Making Equipment Reservations

TGen CSC Service Costs 


Available Services


Search available services: View: by category alphabetically
library preparation (1)

Available Equipment and Resources

Equipment

Name Price